cheR [2019-09-18 09:47:04]

MCPs methyltransferase

Locus: BSU_22720

Isoelectric point: 9.25

Molecular weight: 29.80 kDa

Protein length: 256 aa

Gene length: 771 bp

Function: motility, chemotaxis

Product: MCPs methyltransferase

Essential: no

E.C.: 2.1.1.80

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein modification/ other
• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Soluble signalling proteins

Gene

Coordinates: 2,380,347 2,381,117

The protein

Catalyzed reaction/ biological activity

• methylation of specific glutamate residues in the cytoplasmic domain of methyl-accepting chemotactic protein receptors (MCPRs)
• L-glutamyl-[protein] + S-adenosyl-L-methionine --> [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine (according to UniProt)

Structure

• 5FTW (complex with S-adenosyl-L-homocysteine)

Expression and Regulation

Operons

• Genes: cheR-aroF-aroB-aroH
  Description: PubMed

Biological materials

Mutant

• 1A830 ( cheR::cat), PubMed, available at BGSC
• BKE22720 (cheR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCTACTCTCCCAGTCT, downstream forward: _UP4_TAGACTGACTTTTTGCTGAA
• BKK22720 (cheR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCTACTCTCCCAGTCT, downstream forward: _UP4_TAGACTGACTTTTTGCTGAA

References

Reviews

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Original publications

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